Acta Mechanica Slovaca 2024, 28(2):52-60 | DOI: 10.21496/ams.2024.030

Conceptual Design of a Bioreactor for Tissue Engineering and Regenerative Medicine

Petra Kolembusová1, Norbet Ferenčík1, Radovan Hudák1
Technical University of Košice, Faculty of Mechanical Engineering, Department of Biomedical Engineering, Letná 1/9, 042 00, Košice, Slovak Republic

The aim of the paper was to make an overview of current bioreactor systems in tissue engineering and introduces a conceptual design for a perfusion bioreactor tailored for vascular tissue culture. Perfusion bioreactors were selected for their ability to replicate the in vivo environment, particularly for blood vessels, by providing continuous media flow and applying shear stress, both critical for successful vascular tissue growth. Three bioreactor variants were designed, each incorporating specific modifications aimed at improving the tissue culture process. Variant A consists of a cylindrical chamber that allows for uniform external media flow. Variant B features an hourglass-shaped cultivation chamber, which increases pressure on the cultured tissue, thereby enhancing mechanical stimulation. Variant C, the most promising design, integrates both shear stress and longitudinal stress, aiming to produce vascular tissues with superior mechanical properties. Our results suggest that Variant C holds the greatest potential for achieving robust in vitro vascular tissue formation due to its combined mechanical stimulations. The design prioritizes scalability, ease of assembly, and sterilization, making it suitable for future applications in tissue engineering and regenerative medicine. However, further experimental testing is needed to assess flow dynamics, shear stress, and material performance to refine the bioreactor system and validate its effectiveness in laboratory conditions.

Keywords: bioreactor; stimulation; differentiation; tissue engineering; perfusion; tissue culture

Received: May 6, 2024; Revised: October 18, 2024; Accepted: December 19, 2024; Published: June 14, 2024  Show citation

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Kolembusová, P., Ferenčík, N., & Hudák, R. (2024). Conceptual Design of a Bioreactor for Tissue Engineering and Regenerative Medicine. Acta Mechanica Slovaca, 28(2), 52-60. doi: 10.21496/ams.2024.030
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